CastClark Gregg
PHIL COULSON- CastMing-Na Wen
MELINDA MAY - CastChloe Bennet
SKYE1 / DAISY JOHNSON / QUAKE - CastBrett Dalton
GRANT WARD - CastIain De Caestecker
LEO FITZ - CastElizabeth Henstridge
JEMMA SIMMONS - CastNick Blood
LANCE HUNTER - CastAdrianne Palicki
BOBBI MORSE - CastHenry Simmons
ALPHONSO 'MAC' MACKENZIE - CastLuke Mitchell
LINCOLN CAMPBELL - CastNatalia Cordova-Buckley
ELENA 'YO-YO' RODRIGUEZ - CastBrett Dalton
HIVE - CastJohn Hannah
HOLDEN RADCLIFFE - CastJeff WardDEKE SHAW
- CastClark GreggSARGE / PACHAKUTIQ
- RecurringCobie Smulders
MARIA HILL - RecurringJ. August Richards
MIKE PETERSON1 / DEATHLOK - RecurringRuth Negga
RAINA - RecurringSaffron Burrows
VICTORIA HAND - RecurringBill Paxton
JOHN GARRETT - RecurringAdrian Pasdar
GLENN TALBOT / GRAVITON2 - RecurringPatton Oswalt
ERIC KOENIG - RecurringMaximiliano Hernández
JASPER SITWELL - RecurringKyle MacLachlan
CALVIN JOHNSON (Calvin Zabo) 
RecurringReed Diamond
WERNER REINHARDT1 / DANIEL WHITEHALL- RecurringBrian Patrick Wade
CARL CREEL / L'UOMO ASSORBENTE - RecurringMaya Stojan
KARA LYNN PALAMAS / AGENTE 33 - RecurringMatthew Willig
LASH - RecurringDichen Lachman
JIAYING - RecurringJamie Harris
GORDON - RecurringSpencer Treat Clark
WERNER VON STRUCKER - RecurringAxle Whitehead
J.T. JAMES / HELLFIRE - RecurringMallory Jansen
AIDA - RecurringMallory Jansen
OPHELIA / MADAME HYDRA - RecurringGabriel Luna
ROBBIE REYES / GHOST RIDER - RecurringLorenzo James Henrie
GABE REYES - RecurringJason O’Mara
JEFFREY MACE / PATRIOT - RecurringJosé Zúñiga
ELI MORROW - RecurringZach McGowan
ANTON IVANOV (Red Guardian) - RecurringCoy Stewart
FLINT - RecurringPatrick Warburton
RICK STONER - RecurringJames PaxtonJOHN GARRETT DA GIOVANE
- Guest StarSamuel L. Jackson
NICK FURY - Guest StarIan HartFRANKLIN HALL
- Guest StarLouis Ozawa
CHAN HO YIN1 / SCORCH - Guest StarDylan Minnette
DONNIE GILL - Guest StarJaimie Alexander
LADY SIF - Guest StarElena Satine
LORELEI - Guest StarPatrick Brennan
MARCUS DANIELS - Guest StarLucy Lawless
ISABELLE 'IZZY' HARTLEY - Guest StarHayley Atwell
PEGGY CARTER - Guest StarKenneth Choi
JIM MORITA - Guest StarNeal McDonough
DUM DUM DUGAN - Guest StarFalk Hentschel
MARCUS SCARLOTTI - Guest StarJoel Gretsch
HANK THOMPSON (Cameron Klein) - Guest StarMonique Gabriela Curnen
JANICE ROBBINS (Rebecca Stevens) - Guest StarLou Ferrigno Jr.
HAUER - Guest StarDrea de Matteo
KARLA FAYE GIDEON - Guest StarJeff Daniel Phillips
DAVID ANGAR - Guest StarDaniel Zacapa
CANELO - Guest StarTom McComas
GHOST RIDER - Guest StarArtemis Pebdani
L.T. KOENIG (Ilsa Koenig) - Guest StarJoey Defore
WOLFGANG VON STRUCKER DA GIOVANE - Guest StarRemington Hoffman
MASTON-DAR - Guest StarAdam FaisonJASPER SITWELL DA GIOVANE
- Guest StarRachele SchankVICTORIA HAND DA GIOVANE
- Special, Original & CameoChristine Adams
ANNE WEAVER - Special, Original & CameoTitus WelliverFELIX BLAKE
- Special, Original & CameoPeter MacNicolELLIOT RANDOLPH (1x08, 3x02)
- Special, Original & CameoStan LeeGENTILUOMO DISINVOLTO (1x13)
- Special, Original & CameoHenry GoodmanDR. LIST
- Special, Original & CameoAndrew HowardLUTHER BANKS
- Special, Original & CameoAlex Hyde-WhiteLORD THORNALLY (3x02)
- Special, Original & CameoPowers BootheGIDEON MALICK
- Special, Original & CameoMark DacascosR. GIYERA
- Special, Original & CameoYancey AriasVICTOR RAMON (3x11)
- Special, Original & CameoAlexander WraithANDERSON
- Special, Original & CameoLilli BirdsellLUCY BAUER
- Special, Original & CameoDerek TadlockCECILIO (4x02, 4x13)
- Special, Original & CameoEve HarlowTESS
- Special, Original & CameoPruitt Taylor VinceGRILL
- Special, Original & CameoDove CameronRUBY HALE
- Special, Original & CameoPeter MensahQOVAS
- Special, Original & CameoChristopher James BakerMALACHI
- Special, Original & CameoEnver GjokajDANIEL SOUSA
- Special, Original & CameoTamara TaylorSIBYL
1 Nome originale ideato appositamente per lo show.
2 Il personaggio unisce due figure dell'universo fumettistico Marvel: il colonnello Glenn Talbot e successivamente il criminale Graviton, la cui vera identità di Franklin Hall è stata presentata con un altro personaggio interpretato da Ian Hall.
